Datasheet Blank Template - Santa Cruz Biotechnology

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siRNA, protein, gene, with, Biol., siRNAs, expression, Control, lyophilized, range:, Chem., goat, duplex, dilution, Cruz, 2005., (dilution, include:, Western, receptor, immunophilin, Reagent:, Each, 279:, anti-mouse, RT-PCR, 2004., support, cells., IgG-HRP:


HSP 56 siRNA (h): sc-35602
Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0
HSP 56 (also designated FKBP4, FK506 binding protein 4, HBI, p52, FKBP52,
FKBP59 and PPIase) is a 56 kDa cis-trans prolyl isomerase belonging to the
immunophilin protein family. The human HSP 56 gene (FKBP4) has multiple
polyadenylation sites and the HSP 56 protein can undergo phosphorylation.
HSP 56 influences immunoregulatory gene expression in lymphocytes, protein
folding and trafficking. It can serve as a co-chaperone for steroid hormone
nuclear receptors to govern appropriate hormone action in target tissues.
The protein can associate with phytanoyl-CoA alpha-hydroxylase (PHYH)
and with HSP 90 through a series of tetratricopeptide repeat (TPR) domains.
HSP 56 is a TRPC ion channel accessory protein that modulates channel
activation following receptor stimulation.
1. Sumanasekera, W.K., et al. 2003. Heat shock protein 90 (HSP 90) acts as
a repressor of peroxisome proliferator-activated receptor alpha (PPARα)
and PPARβ activity. Biochemistry 42: 10726-10735.
2. Galigniana, M.D., et al. 2004. HSP 90-binding immunophilins link p53 to
Dynein during p53 transport to the nucleus. J. Biol. Chem. 279: 2248322489.
3. Sanokawa-Akakura, R., et al. 2004. A novel role for the immunophilin
FKBP52 in copper transport. J. Biol. Chem. 279: 27845-27848.
4. Sinkins, W.G., et al. 2004. Association of immunophilins with mammalian
TRPC channels. J. Biol. Chem. 279: 34521-34529.
5. Davies, T.H., et al. 2005. FKBP52. Int. J. Biochem. Cell. Biol. 37: 42-47.
6. Wochnik, G.M., et al. 2005. FK506-binding proteins 51 and 52 differentially
regulate Dynein interaction and nuclear translocation of the glucocorticoid
receptor in mammalian cells. J. Biol. Chem. 280: 4609-4616.
7. Prince, T., et al. 2005. Evidence for chaperone heterocomplexes containing
both HSP 90 and VCP. Biochem. Biophys. Res. Commun. 331: 1331-1337.
8. Tranguch, S., et al. 2005. Co-chaperone immunophilin FKBP52 is critical
to uterine receptivity for embryo implantation. Proc. Natl. Acad. Sci. USA
102: 14326-14331.
Genetic locus: FKBP4 (human) mapping to 12p13.33.
HSP 56 siRNA (h) is a pool of 3 target-specific 19-25 nt siRNAs designed to
knock down gene expression. Each vial contains 3.3 nmol of lyophilized
siRNA, sufficient for a 10 µM solution once resuspended using protocol
below. Suitable for 50-100 transfections. Also see HSP 56 shRNA Plasmid
(h): sc-35602-SH and HSP 56 shRNA (h) Lentiviral Particles: sc-35602-V as
alternate gene silencing products.
For independent verification of HSP 56 (h) gene silencing results, we also
provide the individual siRNA duplex components. Each is available as 3.3 nmol
of lyophilized siRNA. These include: sc-35602A, sc-35602B and sc-35602C.
Store lyophilized siRNA duplex at -20° C with desiccant. Stable for at least
one year from the date of shipment. Once resuspended, store at -20° C,
avoid contact with RNAses and repeated freeze thaw cycles.
Resuspend lyophilized siRNA duplex in 330 µl of the RNAse-free water
provided. Resuspension of the siRNA duplex in 330 µl of RNAse-free water
makes a 10 µM solution in a 10 µM Tris-HCl, pH 8.0, 20 mM NaCl, 1 mM
EDTA buffered solution.
HSP 56 siRNA (h) is recommended for the inhibition of HSP 56 expression in
human cells.
For optimal siRNA transfection efficiency, Santa Cruz Biotechnology’s
siRNA Transfection Reagent: sc-29528 (0.3 ml), siRNA Transfection Medium:
sc-36868 (20 ml) and siRNA Dilution Buffer: sc-29527 (1.5 ml) are recommended. Control siRNAs or Fluorescein Conjugated Control siRNAs are
available as 10 µM in 66 µl. Each contain a scrambled sequence that will
not lead to the specific degradation of any known cellular mRNA. Fluorescein
Conjugated Control siRNAs include: sc-36869, sc-44239, sc-44240 and
sc-44241. Control siRNAs include: sc-37007, sc-44230, sc-44231, sc-44232,
sc-44233, sc-44234, sc-44235, sc-44236, sc-44237 and sc-44238.
HSP 56 (329.1): sc-100758 is recommended as a control antibody for monitoring of HSP 56 gene expression knockdown by Western Blotting (starting dilution 1:200, dilution range 1:100-1:1000) or immunofluorescence (starting dilution 1:50, dilution range 1:50-1:500).
To ensure optimal results, the following support (secondary) reagents are
recommended: 1) Western Blotting: use goat anti-mouse IgG-HRP: sc-2005
(dilution range: 1:2000-1:32,000) or Cruz Marker™ compatible goat antimouse IgG-HRP: sc-2031 (dilution range: 1:2000-1:5000), Cruz Marker™
Molecular Weight Standards: sc-2035, TBS Blotto A Blocking Reagent:
sc-2333 and Western Blotting Luminol Reagent: sc-2048. 2) Immunofluorescence: use goat anti-mouse IgG-FITC: sc-2010 (dilution range: 1:1001:400) or goat anti-mouse IgG-TR: sc-2781 (dilution range: 1:100-1:400)
with UltraCruz™ Mounting Medium: sc-24941.
Semi-quantitative RT-PCR may be performed to monitor HSP 56 gene expression knockdown using RT-PCR Primer: HSP 56 (h)-PR: sc-35602-PR (20 µl).
Annealing temperature for the primers should be 55-60° C and the extension
temperature should be 68-72° C.
For research use only, not for use in diagnostic procedures.
See our web site at or our catalog for detailed protocols and
support products.

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