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Keywords

ARK5, dilution, cell, range:, (dilution, anti-goat, donkey, Western, 0.1%, factor, which, protein, range, (starting, (h):, recommended, peptide, human, (N-14), Cruz, detection, regulatory, tumor, Reagent:, Marker™, mapping, IgG-HRP:, with, support, sodium

Transcript

SANTA CRUZ BIOTECHNOLOGY, INC.
ARK5 (N-14): sc-49366
Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com
BACKGROUND
5-prime-AMP-activated protein kinase, known as AMPK, is a heterotrimeric
complex that protects cells from stresses which cause ATP depletion by
switching off ATP-consuming biosynthetic pathways. Activated AMPK phosphorylates and regulates hydroxymethylglutaryl-CoA reductase and acetylCoA carboxylase, which are key regulatory enzymes of sterol synthesis and
fatty acid synthesis, respectively. The AMPK-related kinase 5 (ARK5) is a
tumor progression-associated factor that is directly phosphorylated by AKT
at Serine 600, which is in its regulatory domain. NDR2, a serine/threonine
protein kinase, phosphorylates and activates ARK5 during Insulin-like growth
factor (IGF)-1 signaling in order to promote cell survival. This activation may
be involved in the invasion of colorectal cancer cell lines. Furthermore, ARK5
is a transcriptional target of the large-Maf family and may have a role in
mediating the aggressiveness of c-Maf- and MafB-expressing myelomas.
REFERENCES
1. Suzuki, A., et al. 2003. ARK5 suppresses the cell death induced by nutrient
starvation and death receptors via inhibition of caspase 8 activation, but
not by chemotherapeutic agents or UV irradiation. Oncogene 22: 6177-6182.
2. Suzuki, A., et al. 2004. ARK5 is a tumor invasion-associated factor downstream of Akt signaling. Mol. Cell. Biol. 24: 3526-3535.
CHROMOSOMAL LOCATION
Genetic locus: NUAK1 (human) mapping to 12q23.3.
SOURCE
ARK5 (N-14) is an affinity purified goat polyclonal antibody raised against a
peptide mapping near the N-terminus of ARK5 of human origin.
PRODUCT
Each vial contains 200 µg IgG in 1.0 ml of PBS with < 0.1% sodium azide
and 0.1% gelatin.
Blocking peptide available for competition studies, sc-49366 P, (100 µg
peptide in 0.5 ml PBS containing < 0.1% sodium azide and 0.2% BSA).
APPLICATIONS
ARK5 (N-14) is recommended for detection of ARK5 of human origin by Western Blotting (starting dilution 1:200, dilution range 1:100-1:1000), immunoprecipitation [1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilution range 1:50-1:500) and solid
phase ELISA (starting dilution 1:30, dilution range 1:30-1:3000).
ARK5 (N-14) is also recommended for detection of ARK5 in additional
species, including canine, bovine, porcine and avian.
Suitable for use as control antibody for ARK5 siRNA (h): sc-60203, ARK5
shRNA Plasmid (h): sc-60203-SH and ARK5 shRNA (h) Lentiviral Particles:
sc-60203-V.
Molecular Weight of ARK5: 74 kDa.
Positive Controls: ARK5 (h): 293T Lysate: sc-173768.
RECOMMENDED SECONDARY REAGENTS
To ensure optimal results, the following support (secondary) reagents are
recommended: 1) Western Blotting: use donkey anti-goat IgG-HRP: sc-2020
(dilution range: 1:2000-1:100,000) or Cruz Marker™ compatible donkey
anti-goat IgG-HRP: sc-2033 (dilution range: 1:2000-1:5000), Cruz Marker™
Molecular Weight Standards: sc-2035, TBS Blotto A Blocking Reagent:
sc-2333 and Western Blotting Luminol Reagent: sc-2048. 2) Immunoprecipitation: use Protein A/G PLUS-Agarose: sc-2003 (0.5 ml agarose/2.0 ml).
3) Immunofluorescence: use donkey anti-goat IgG-FITC: sc-2024 (dilution
range: 1:100-1:400) or donkey anti-goat IgG-TR: sc-2783 (dilution range:
1:100-1:400) with UltraCruz™ Mounting Medium: sc-24941.
DATA
STORAGE
Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of
shipment. Non-hazardous. No MSDS required.
RESEARCH USE
For research use only, not for use in diagnostic procedures.
PROTOCOLS
See our web site at www.scbt.com or our catalog for detailed protocols
and support products.
Try ARK5 (A-9): sc-271827 or ARK5 (B-4): sc-271828,
our highly recommended monoclonal alternatives to
ARK5 (N-14).
ARK5 (N-14): sc-49366. Western blot analysis of ARK5
expression in non-transfected: sc-117752 (A) and
human ARK5 transfected: sc-173768 (B) 293T whole
cell lysates.
132 K –
90 K –
55 K –
A B
ARK5

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