Product Data Sheet - BioLegend

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Version: 5 Revision Date: 2015-07-13
Product Data Sheet
PerCP anti-human CD8a
Human peripheral blood lymphocytes
stained with HIT8a PerCP
Catalog # / Size: 300921 / 25 tests
300922 / 100 tests
Clone: HIT8a
Isotype: Mouse IgG1, κ
Workshop Number: V CD08.10
Reactivity: Human, Cross-Reactivity: Chimpanzee, Marmoset
Preparation: The antibody was purified by affinity chromatography, and conjugated
with PerCP under optimal conditions. The solution is free of unconjugated
PerCP and unconjugated antibody.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and
0.2% (w/v) BSA (origin USA).
Concentration: Lot-specific (please contact technical support for concentration and total
µg amount, or use our Lookup tool if you have a lot number.)
Storage: The antibody solution should be stored undiluted between 2°C and 8°C,
and protected from prolonged exposure to light. Do not freeze.
Applications:
Applications: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow
cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended
that the reagent be titrated for optimal performance for each application.
* PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.
Application Notes: Clone HIT8a recognizes the alpha chain of CD85. It does not block the binding of RPA-T8 antibody to CD8a.
Additional reported applications of this antibody (for the relevant formats) include: immunohistochemical staining of
acetone-fixed frozen tissue sections5,6. This clone was tested in-house and does not work on formalin fixed
paraffin-embedded (FFPE) tissue.
Application References: 1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
2. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
3. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
4. Awasthi, S., et al. 2011. J. Virol 85:10472. PubMed
5. Coppieters KT, et al. 2012. J. Exp. Med. 209:51. (IHC, epitope)
6. Suzuki F, et al. 2012. Arthritis Res. Ther. 14:R48. (IHC)
RRID: AB_1575076 (BioLegend Cat. No. 300921)
AB_1575072 (BioLegend Cat. No. 300922)
Description: CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8,
also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a
subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a
co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been
shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF
domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.
Other Names: T8, Leu2
Antigen References: 1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.
Related Products:Product Clone Application
PerCP Mouse IgG1, κ Isotype Ctrl MOPC-21 FC, ICFC
Cell Staining Buffer FC, ICC, ICFC
RBC Lysis Buffer (10X) FC, ICFC
Human TruStain FcX (Fc Receptor Blocking Solution) FC, ICC, ICFC
For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible
for patent infringement or other violations that may occur with the use of our products.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may
not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third
parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these
products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
BioLegend, Inc., 9727 Pacific Heights Blvd, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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